SERCA Dynamic Structures Characterized with FLDA-FRET Method
نویسندگان
چکیده
منابع مشابه
High-throughput FRET assay yields allosteric SERCA activators.
Using fluorescence resonance energy transfer (FRET), we performed a high-throughput screen (HTS) in a reconstituted membrane system, seeking compounds that reverse inhibition of sarcoplasmic reticulum Ca-ATPase (SERCA) by its cardiac regulator, phospholamban (PLB). Such compounds have long been sought to correct aberrant Ca(2+) regulation in heart failure. Donor-SERCA was reconstituted in phosp...
متن کاملNucleosome disassembly intermediates characterized by single-molecule FRET.
The nucleosome has a central role in the compaction of genomic DNA and the control of DNA accessibility for transcription and replication. To help understanding the mechanism of nucleosome opening and closing in these processes, we studied the disassembly of mononucleosomes by quantitative single-molecule FRET with high spatial resolution, using the SELEX-generated "Widom 601" positioning seque...
متن کاملTime-resolved FRET reveals the structural mechanism of SERCA-PLB regulation.
We have used time-resolved fluorescence resonance energy transfer (TR-FRET) to characterize the interaction between phospholamban (PLB) and the sarcoplasmic reticulum (SR) Ca-ATPase (SERCA) under conditions that relieve SERCA inhibition. Unphosphorylated PLB inhibits SERCA in cardiac SR, but inhibition is relieved by either micromolar Ca(2+) or PLB phosphorylation. In both cases, it has been pr...
متن کاملVisualizing dynamic cytoplasmic forces with a compliance-matched FRET sensor.
Mechanical forces are ubiquitous modulators of cell activity but little is known about the mechanical stresses in the cell. Genetically encoded FRET-based force sensors now allow the measurement of local stress in specific host proteins in vivo in real time. For a minimally invasive probe, we designed one with a mechanical compliance matching that of many common cytoskeleton proteins. sstFRET i...
متن کاملDynamic Analysis of Cylindrically Layered Structures Reinforced by Carbon Nanotube Using MLPG Method
This paper deals with the dynamic analysis of stress field in cylindrically layeredstructures reinforced by carbon nanotube (CLSRCN) subjected to mechanical shock loading.Application of meshless local integral equations based on meshless local Petrov-Galerkin(MLPG) method is developed for dynamic stress analysis in this article. Analysis is carriedout in frequency domain by applying the Laplace...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2013
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2012.11.372